Biotinylated Polyclonal Antibodies
Biotinylated Anti-Murine TNF-α





 





		Preparation :
Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant mTNF-α.  Anti-Murine TNF-α specific antibody was purified by affinity chromatography and then biotinylated.



		Catalog #:
500-P64Bt



		Source :
Rabbits



		Formulation :
A sterile filtered antibody solution was lyophilized from PBS, pH 7.2.



		Stability :
The lyophilized antibody is stable for at least 2 years from date of receipt at -200C. The reconstituted antibody is stable for at least two weeks at 2-80C. Frozen aliquots are stable for at least 6 months when stored at -200C.  Avoid repeated freeze-thaw cycles.


Reconstitution:
Centrifuge vial prior to opening. Reconstitute in sterile PBS containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml.



		Applications :
 


Selected References :
Selected List of References Citing This PeproTech Product



		Elisa 
Direct:
To detect mTNF-α by direct ELISA (using 100 μl/well antibody solution) a concentration of 0.25 C 1.0 μg/ml of this antibody is required.  This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 C 0.4 ng/well of recombinant mTNF-α.

Sandwich:
To detect mTNF-α by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.25 C 1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech’s Polyclonal Anti-Murine TNF-α (500-P64) as a capture antibody, allows the detection of at least 0.2 C 0.4 ng/well of recombinant mTNF-α.



		Western Blot 
To detect mTNF-α by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mTNF-α is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.


Country of Origin :
USA
















        Size A
25μg
$195 
 


 



        Size B
50μg
$250 
 


 



        Size C
1mg
$4000 
 


 















Special 
                    Sizes: 
 Many products can be ordered in custom sizes. Please click here to contact Peprotech with your request.





Usage:
For Research Use Only.  Not for use in diagnostic or therapeutic procedures.