Product: Anti-Chk2 pAb
Cat. No.: PC-530 (100 μg)
Synonyms:
Anti-Checkpoint Kinase 2.
Background:
The p53 tumor-suppressor gene integrates
numerous signals that control cell life and death.
Several novel molecules involved in p53
signaling, including Chk2, p53R2, p53AIP1,
Noxa, PIDD, and PID/MTA2, were recently
discovered. The checkpoint kinase Chk2 is the
mammalian homologue of yeast Cds1/Rad53. In
response to DNA damage, the checkpoint
kinase ATM phosphorylates and activates Chk2,
which in turn directly phosphorylates and
activates p53. Chk2 serves as ATM downstream
effector to mediate activation of p53. Chk2 also
phosphorylates and activates BRCA1, the
product of a tumor suppressor gene that is
mutated in breast and ovarian cancer.
Specificity:
Recognizes Chk2.
Positive Controls:
Jurkat or K562 cell lysates. A 60 kDa band can
be detected.
Species Reactivity:
Human, mouse and rat. Other species not tested.
Host:
Rabbit
Immunogen:
A synthetic peptide corresponding to amino
acids near the amino-terminus of human Chk2.
Format:
100 μg of polyclonal antibody in PBS containing
0.02% sodium azide. Purified by immunoaffinity
chromatography.
Storage:
Store at 4°C.
Applications and Suggested Dilutions:

Western blot: Use at 0.5 - 1 μg/mL.

Immunocytochemistry: Use at ~1 μg/mL.
The optimal dilution for a specific application
should be determined by the researcher.
Figure 1: Western blot analysis of Chk2 expression in
K562 (A), Jurkat (B), and HL-60 (C) whole cell lysates
at 1 μg/mL.
Figure 2: Immunocytochemistry of Chk2 in Jurkat
cells with anti-Chk2 pAb at 1 μg/mL.
Limitations:
For in vitro research use only. Not for use in
diagnostics or in humans.
Warranty:
No warranties, expressed or implied, are made
regarding the use of this product. KAMIYA
BIOMEDICAL COMPANY is not liable for any
damage, personal injury, or economic loss
caused by this product